Mark Wilson and Anthony Watson replace Sam Underhill and Jonathan Joseph respectively in the England side to face Wales.
Joe Cokanasiga, Jonathan Joseph and Lewis Ludlam have come into the England team due to a trio of injuries.
British and Irish Lions duo Maro Itoje and Jonathan Joseph, as well as former skipper Chris Robshaw, have been called up by England.
Reported Northampton Saints target Jonathan Joseph is set to stay at Bath after signing an undisclosed contract extension.
England's options for their tour of South Africa are likely to be further restricted, with Jonathan Joseph ruled out for the season.