Injuries sustained by Garry Ringrose and Caelan Doris open the door for Robbie Henshaw and Peter O'Mahony to start against Wales.
Ireland coach Joe Schmidt has brought Rob Kearney back to face New Zealand, while Garry Ringrose will partner Robbie Henshaw at centre.
Peter O'Mahony is relishing the prospect of leading Ireland out in Rome in the absence of the rested Rory Best.
A rare Aviva Stadium defeat was painful for Ireland, yet Peter O'Mahony said: "We can't be hanging around feeling sorry for ourselves."
Joey Carbery, Rory Scannell, Keith Earls and Andrew Conway were among the tries as Munster overpowered Gloucester.
Peter O'Mahony knows it would be a major achievement to mark his 50th cap by inspiring Ireland to a series win over Australia.
Michael Cheika was not impressed with Neil Francis' choice of words when criticising David Pocock and neither was Peter O'Mahony.
Australia did not always have it their own way against a much-changed Ireland team, but, according to Michael Hooper, that was their plan.
Ireland skipper Peter O'Mahony expects Australia to provide the same tough test they usually do, resulting in a "huge battle".