Italy have had a second Six Nations match postponed due to the coronavirus, with doubts over whether the tournament will be completed.
Mark Wilson and Anthony Watson replace Sam Underhill and Jonathan Joseph respectively in the England side to face Wales.
Following a decree by Italy's prime minister, England's final Six Nations match of 2020 looks set to be played without fans in attendance.
Consistency of performance is what England have been lacking in the Six Nations, according to World Cup-winning captain Martin Johnson.
Mako Vunipola could play for Saracens this weekend, but England were advised not to take any risks following the prop's trip overseas.
Chris Ashton has returned to London to join Harlequins after his Sale Sharks contract was terminated.
Saracens have clarified Mako Vunipola has not been in self-isolation and is free to play Leicester Tigers this weekend.
Experienced duo Dan Biggar and Liam Williams are on course to be fit for Wales' showdown with England at Twickenham.
Initially ruled out of the Wales Six Nations game for "medical reasons", England confirmed Mako Vunipola is in self-isolation.
England will not have Mako Vunipola back to face Wales, with the prop ruled out for the Six Nations encounter at Twickenham.
Ireland's clash with Italy in Dublin is the only Six Nations match to be postponed due to the coronavirus.
Nemani Nadolo will be in Leicester Tigers colours next season after the Premiership club lured the former Fiji powerhouse from Montpellier.
England prop Mako Vunipola features in their 34-man squad ahead of the game with Wales, with Jack Maunder called up.
Chris Ashton is on the market after Premiership contenders Sale Sharks reached an agreement with the wing to terminate his contract early.
Fly-half Finn Russell will play no part in the Six Nations, but could make his Scotland return later this year.
Ben Botica scored two second-half penalties to help Bordeaux Begles to a 26-24 victory in a tightly contested match against Castres.