Scotland have called up the fit-again Sam Skinner and Matt Fagerson ahead of their clash with Italy in Rome on February 22.
Finn Russell looks unlikely to play for Scotland under Gregor Townsend after a statement released by the head coach on Monday.
Scotland will be without Jonny Gray for the rest of the Six Nations as he will return to Glasgow Warriors for treatment on an injury.
Finn Russell has criticised head coach Gregor Townsend and said "it's not really possible for me to be involved" for Scotland.
England coach Eddie Jones was far from impressed with the behaviour of some Scotland supporters as England regained the Calcutta Cup.
Prop Ellis Genge said "I hope we have shut them up" as he laid into England's critics after they beat Scotland.
Stuart Hogg made another costly mistake as England beat Scotland in an ugly Calcutta Cup clash to claim their first win of the Six Nations.
Captain Stuart Hogg is confident Scotland can let their rugby do the talking and beat England to retain the Calcutta Cup.
Gregor Townsend will consider Finn Russell's involvement in the Six Nations after the Calcutta Cup clash on Saturday.
After both recorded opening wins, Ireland and Wales clash in Dublin. Meanwhile, there's the small matter of a Calcutta Cup clash.
Scotland have resisted the temptation to make sweeping changes for the Calcutta Cup contest with England.
Eddie Jones has rung the changes for England's crucial Calcutta Cup contest with Scotland at Murrayfield.
Finn Russell will miss his second straight game when Scotland take on England, but Blair Kinghorn spoke of the squad's confidence.
England were humbled by France in their Six Nations opener and Eddie Jones could be set to make changes for the clash with Scotland.
England must show they have a mature mentality if they are to defeat Scotland and clinch the Calcutta Cup, says Courtney Lawes.
England will again have to cope without Anthony Watson as they look to bounce back from defeat to France in their Six Nations opener.